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器官型切片文化绿色荧光蛋白表达的实时成像周围神经生长的小鼠胚胎
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
Please note that all translations are automatically generated.
Click here for the English version.
器官型切片文化绿色荧光蛋白表达的实时成像周围神经生长的小鼠胚胎
DOI:
10.3791/2309-v
•
05:42 min
•
March 29, 2011
•
Isabel Brachmann
,
Kerry L. Tucker
1
Interdisciplinary Center for Neurosciences, Institute of Anatomy and Cell Biology
,
University of Heidelberg
Chapters
00:05
Title
01:20
Preparation for Slicing and Culturing
02:08
Embryo Embedding
03:18
Slicing Procedure
04:23
Imaging Spinal Nerve Outgrowth
04:54
Representative Results of Imaging Spinal Nerve Outgrowth
05:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们现在准备妊娠中期的小鼠胚胎的器官切片周围神经生长的培养和时间推移成像的方法。
Tags
Organotypic Slice Culture
GFP-expressing Mouse Embryos
Real-time Imaging
Peripheral Nerve Outgrowth
Ex Vivo Cultivation
Transgenic Mouse Line
TauGFP
Green Fluorescent Protein
Neurons
Central Nervous System
Peripheral Nervous System
Forelimb Innervation
Pharmacological Techniques
Genetic Techniques
Vibratome
Slice Preparation
Viability
Age-specific Development
Spinal Nerves
Spinal Cord
Dorsal Root Ganglia
Embryonic Day (E) E10 To E12 Slices
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