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Chapters
- 00:05Title
- 01:19Pericam-mt Transfection and Cell Preparation
- 04:11Image Processing and Analysis
- 04:37Fluorescence Imaging Results
- 05:58Conclusion
Questo protocollo descrive un metodo per la misurazione in tempo reale dei flussi di calcio mitocondriale di imaging fluorescente. Il metodo si avvale di una circolare permutated YFP a base di sensori di calcio dual-eccitazione raziometrico (raziometrico pericam-mt) l'indicazione espressa nei mitocondri.
Tags
MonitoringDynamic ChangesMitochondrial Calcium LevelsApoptosisGenetically Encoded Calcium SensorIntracellular Calcium ConcentrationStimuliCellular ProcessesProliferationDifferentiationCalcium AccumulationPro-apoptotic FactorsCytosolLiving CellsIn SituHigh-resolution Fluorescent ImagingDual-excitation RatiometricNon-ratiometric Synthetic Calcium Indicator DyesIntracellular Calcium SignalingCytosolic Calcium FluxesRhod-2Rhod-FFSynthetic IndicatorsMitochondriaRepetitive IncreasesMitochondrial MorphologyLeak Out Of MitochondriaLong-term ExperimentsGenetically Encoded Calcium IndicatorsGreen Fluorescent Protein (GFP)Aequorin
