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一个视神经挤压伤的小鼠模型,研究视网膜神经节细胞生存
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
DOI:

09:07 min

April 25, 2011

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Chapters

  • 00:05Title
  • 02:25Baseline Retrograde Labeling of Retinal Ganglion Cells (RGCs) at Day 1
  • 04:47Optic Nerve Crush Injury at Day 4 after Fluorescence Dye Injection
  • 06:22Retinal Harvest and Analysis of RGC Survival
  • 07:13Analysis of Retinal Ganglion Cell Survival after Optic Nerve Crush Injury
  • 08:01Conclusion

Summary

Automatic Translation

该协议显示了如何逆行标记视网膜神经节细胞,以及如何随后视神经挤压伤,以分析视网膜神经节细胞的存活和凋亡。这是一个为不同类型的视神经病变,包括青光眼的实验性疾病模型。

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