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Journal
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Immunology and Infection
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钩端螺旋体暴露于表面的蛋白免疫荧光法
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Please note that all translations are automatically generated.
Click here for the English version.
钩端螺旋体暴露于表面的蛋白免疫荧光法
DOI:
10.3791/2805-v
•
10:47 min
•
July 01, 2011
•
Marija Pinne
2
,
David Haake
4
1
Department of Medicine, David Geffen School of Medicine
,
University of California, Los Angeles
,
2
Research service, 151
,
Veterans Affairs Greater Los Angeles Healthcare System
,
3
Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics
,
University of California Los Angeles (UCLA)
,
4
Division of Infectious Diseases, 111F
,
Veterans Affairs Greater Los Angeles Health Care System
Chapters
00:05
Title
02:20
Fixation of Leptospira to Glass Slides
04:51
Labeling with Specific Antibodies
06:33
Visualization of Leptospires
08:42
Data Analysis and Representative Results
10:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一种有效的方法来评估钩端螺旋体蛋白质表面暴露描述。该方法是专门设计,以避免破坏脆弱的钩体细胞的外膜。这种技术需要雇用几个阴性对照,以评估外膜的完整性和特异性抗体反应。
Tags
Immuno-fluorescence Assay
Leptospiral Surface-exposed Proteins
Bacterial Surface Proteins
Cellular Localization
Functional Role
Virulence Factors
Pathogenicity Mechanisms
Fractionating Leptospiral Membranes
Outer-membrane Proteins (OMPs)
Lipoproteins
Transmembrane OMPs
Hydrophobic Interaction
Lipid Bilayer Phospholipids
Amphipathic β-sheets
Barrel-like Structure
Outer Membrane Integrity
Immunofluorescence Assay (IFA)
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