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JoVE Journal Biology
Measuring the Kinetics of mRNA Transcription in Single Living Cells
 

Measuring the Kinetics of mRNA Transcription in Single Living Cells

Article doi: 10.3791/2898
August 25th, 2011 • Usage Statistics
1, 1
1The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University

Summary
Automatic Translation
August 25th, 2011

RNA polymerase II transcriptional kinetics are measured on specific genes in living cells. mRNAs transcribed from the gene of interest are fluorescently tagged and using Fluorescence Recovery After Photobleaching (FRAP) the in vivo kinetics of transcriptional elongation are obtained.

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