Biology
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Measuring the Kinetics of mRNA Transcription in Single Living Cells
Chapters
Summary August 25th, 2011
RNA polymerase II transcriptional kinetics are measured on specific genes in living cells. mRNAs transcribed from the gene of interest are fluorescently tagged and using Fluorescence Recovery After Photobleaching (FRAP) the in vivo kinetics of transcriptional elongation are obtained.
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