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测量单个活细胞中的mRNA转录的动力学
JoVE Journal
Biology
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JoVE Journal
Biology
Measuring the Kinetics of mRNA Transcription in Single Living Cells
Please note that all translations are automatically generated.
Click here for the English version.
测量单个活细胞中的mRNA转录的动力学
DOI:
10.3791/2898-v
•
11:22 min
•
August 25, 2011
•
Yehuda Brody
,
Yaron Shav-Tal
1
The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology
,
Bar-Ilan University
Chapters
00:05
Title
02:33
Tagging of mRNA in Living Cells
04:55
FRAP Experiment
08:10
Data Analysis
09:44
Results of Measuring mRNA Transcription Kinetics
10:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
在活细胞中的特定基因上RNA聚合酶II转录的动力学测量。感兴趣的基因转录的mRNA荧光标记,并使用荧光漂白后(FRAP)在体内转录伸长动力学得到恢复。
Tags
Kinetics
MRNA Transcription
Living Cells
RNA Polymerase II
Transcriptional Activity
Visualization Methods
Analysis
Elongation Kinetics
Gene Locus
Fluorescent Labeling
MS2 System
MRNA Transcripts
YFP-MS2 Coat Protein
Fluorescence Recovery After Photobleaching (FRAP) Method
Synthesis Rate
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