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Medicine
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腭扩展口腔生物膜分析,荧光原位杂交和激光共聚焦显微镜
JoVE Journal
Medicine
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JoVE Journal
Medicine
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
腭扩展口腔生物膜分析,荧光原位杂交和激光共聚焦显微镜
DOI:
10.3791/2967-v
•
09:44 min
•
October 20, 2011
•
Barbara Klug
2
,
Claudia Rodler
,
Martin Koller
,
Gernot Wimmer
,
Harald H. Kessler
,
Martin Grube
,
Elisabeth Santigli
1
Department of Orthodontics and Maxillofacial Orthopedics
,
Medical University of Graz
,
2
Institute of Hygiene, Microbiology and Environmental Medicine
,
Medical University of Graz
,
3
Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology
,
Medical University of Graz
,
4
Institute of Plant Sciences
,
Karl-Franzens-University Graz
Chapters
00:05
Title
01:51
Specimen Collection and Biofilm Fixation
03:38
Dehydration of Fixed Samples
04:34
In-situ Hybridization and Imaging
07:21
Results: Biofilm Images
08:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们目前的协议矫治器的结构和成分分析的天然口腔生物膜与<em>原位</em>杂交(FISH)和共聚焦激光扫描显微镜(CLSM)。口腔生物膜样品分别采自腭扩展,刮关闭其表面的丙烯酸树脂片和指分子处理。
Tags
Oral Biofilm Analysis
Palatal Expanders
Fluorescence In Situ Hybridization (FISH)
Confocal Laser Scanning Microscopy (CLSM)
Staining Techniques
16S RRNA-targeting Probes
Biofilm Consortia
Eubacteria
Firmicutes
Bacteroidetes
Streptococcus Mutans
Porphyromonas Gingivalis
Surface Materials
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