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腭扩展口腔生物膜分析，荧光原位杂交和激光共聚焦显微镜

JoVE Journal
Medicine

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JoVE Journal Medicine

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Please note that all translations are automatically generated. Click here for the English version.

腭扩展口腔生物膜分析，荧光原位杂交和激光共聚焦显微镜

DOI:

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09:44 min

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October 20, 2011

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Barbara Klug², Claudia Rodler, Martin Koller, Gernot Wimmer, Harald H. Kessler, Martin Grube, Elisabeth Santigli

¹Department of Orthodontics and Maxillofacial Orthopedics,Medical University of Graz, ²Institute of Hygiene, Microbiology and Environmental Medicine,Medical University of Graz, ³Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology,Medical University of Graz, ⁴Institute of Plant Sciences,Karl-Franzens-University Graz

Chapters

00:05Title
01:51Specimen Collection and Biofilm Fixation
03:38Dehydration of Fixed Samples
04:34In-situ Hybridization and Imaging
07:21Results: Biofilm Images
08:56Conclusion

Summary

Automatic Translation

我们目前的协议矫治器的结构和成分分析的天然口腔生物膜与<em>原位</em>杂交（FISH）和共聚焦激光扫描显微镜（CLSM）。口腔生物膜样品分别采自腭扩展，刮关闭其表面的丙烯酸树脂片和指分子处理。

Tags

Oral Biofilm Analysis Palatal Expanders Fluorescence In Situ Hybridization (FISH)Confocal Laser Scanning Microscopy (CLSM)Staining Techniques 16S RRNA-targeting Probes Biofilm Consortia Eubacteria Firmicutes Bacteroidetes Streptococcus Mutans Porphyromonas Gingivalis Surface Materials

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