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高活性酵母核糖体的色谱分离纯化
JoVE Journal
Biology
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JoVE Journal
Biology
Chromatographic Purification of Highly Active Yeast Ribosomes
Please note that all translations are automatically generated.
Click here for the English version.
高活性酵母核糖体的色谱分离纯化
DOI:
10.3791/3214-v
•
09:13 min
•
October 24, 2011
•
Arturas Meskauskas
2
,
Jonathan A. Leshin
,
Jonathan D. Dinman
1
Department of Cell Biology and Molecular Genetics
,
University of Maryland
,
2
Department of Biotechnology and Microbiology
,
Vilnius University
Chapters
00:05
Title
01:17
Preparation of a Cysteine Charged Sulfolink Resin
02:36
Chromatographic Purification of Ribosomes Using Cysteine Charged Sulfolink Resin
05:18
Puromycin Treatment & Purification of Ribosomes by Sedimentation Through Glycerol Cushions
07:28
Representative Purification of Highly Active Yeast Ribosomes
08:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
准备用传统的方法,纯化共同净化核酸酶和蛋白酶的真核细胞的核糖体的污染造成了消极影响下游的生化和结构分析。一个快速和简单的色谱的净化方法是用来解决这个问题作为一个模型系统中使用的酵母核糖体。
Tags
Chromatographic Purification
Yeast Ribosomes
Labile Ribosomes
Proteases
Nucleases
Differential Ultracentrifugation
Structural Integrity
Functionality
Cysteine Charged Sulfolink Resin
Co-purifying Proteolytic And Nucleolytic Activities
Intact Ribosomes
Biochemically Active Ribosomes
Mammalian Ribosomes
Technical Advancement
Article
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