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Chapters
- 00:05Title
- 01:17Preparation of a Cysteine Charged Sulfolink Resin
- 02:36Chromatographic Purification of Ribosomes Using Cysteine Charged Sulfolink Resin
- 05:18Puromycin Treatment & Purification of Ribosomes by Sedimentation Through Glycerol Cushions
- 07:28Representative Purification of Highly Active Yeast Ribosomes
- 08:48Conclusion
Forurening af præparater af eukaryote ribosomer renses ved traditionelle metoder ved co-rensende nucleaser og proteaser negativt virkninger på downstream-biokemiske og strukturelle analyser. En hurtig og enkel kromatografisk oprensning metoden anvendes til at løse dette problem ved hjælp af gær ribosomer som model system.
Tags
Chromatographic PurificationYeast RibosomesLabile RibosomesProteasesNucleasesDifferential UltracentrifugationStructural IntegrityFunctionalityCysteine Charged Sulfolink ResinCo-purifying Proteolytic And Nucleolytic ActivitiesIntact RibosomesBiochemically Active RibosomesMammalian RibosomesTechnical Advancement
