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A Protocol for Computer-Based Protein Structure and Function Prediction
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A Protocol for Computer-Based Protein Structure and Function Prediction

A Protocol for Computer-Based Protein Structure and Function Prediction

DOI:
10.3791/3259-v

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16:41 min

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November 03, 2011

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, , ,
1Center for Computational Medicine and Bioinformatics,University of Michigan , 2Center for Bioinformatics and Department of Molecular Bioscience,University of Kansas

Chapters

  • 00:05Title
  • 02:21Running the I-TASSER Server
  • 03:37Structure Analysis
  • 05:58LOMETS Target Template Alignment
  • 07:30Structural Analogs in PDB and Enzyme Commission Number Prediction
  • 09:20Gene Ontology (GO) Term and Protein-ligand Bind site Predictions
  • 12:05Representative I-TASSER Results
  • 15:43Conclusion

Summary

Automatic Translation

Automatic Translation

Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.

Tags

ProtocolComputer-basedProtein StructureFunction PredictionGenome Sequencing ProjectsProtein SequenceStructure And Function KnowledgeExperimental MethodsComputational ModelingI-TASSER ServerSecondary Structure PredictionSolvent Accessibility PredictionHomologous Template ProteinsStructure AlignmentsTertiary Structural ModelsStructure-based Functional AnnotationsConfidence ScoreEnd UsersUser-specified Inter-residue DistanceContact MapsI-TASSER ModelingTemplate ProteinsStructure Assembly Simulations

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