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Journal
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Biology
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冰帽:为发展方法
拟南芥
和番茄植株在96孔板高通量的基因分型
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Ice-Cap: A Method for Growing
Arabidopsis
and Tomato Plants in 96-well Plates for High-Throughput Genotyping
Please note that all translations are automatically generated.
Click here for the English version.
冰帽:为发展方法
拟南芥
和番茄植株在96孔板高通量的基因分型
DOI:
10.3791/3280-v
•
11:15 min
•
November 09, 2011
•
Shih-Heng Su
,
Katie A. Clark
,
Nicole M. Gibbs
,
Susan M. Bush
,
Patrick J. Krysan
1
Horticulture Department
,
University of Wisconsin-Madison
,
2
Department of Zoology
,
Oregon State University
Chapters
00:05
Title
02:04
Preparation of Ice-Cap Seedling Plates with Agar Growth Media
02:53
Sowing and Germination of Seedlings in Ice-Cap Seedling Plates
03:52
Transfer of Seedling Plates to the Ice-Cap Fountain
05:46
Harvesting of Root Tissue
07:51
DNA Extraction
09:19
Planting of Seedlings with the Desired Genotyping
10:14
Representative Results Illustrating Arabidopsis Seedlings Grown Using the Ice-Cap System
10:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
冰帽的方法使一个成长中的96孔板,每个幼苗根组织非破坏性的收获的植物。从这个根组织中提取的DNA可用于基因分型反应。我们已经发现,冰帽可以很好地用于<em>拟南芥</em>,番茄,水稻秧苗。
Tags
Ice-Cap Method
Arabidopsis
Tomato Plants
96-well Plates
High-throughput Genotyping
Bottleneck
Genotyping Pipeline
High-throughput Solution
Seedlings
Agar Growth Media
DNA Extraction
Rapid Freezing
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