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的方向转变,并在电脉冲电场方向的应用,提高质粒的基因转移
在体外</em
JoVE Journal
Medicine
Author Produced
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JoVE Journal
Medicine
Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer
in vitro
Please note that all translations are automatically generated.
Click here for the English version.
的方向转变,并在电脉冲电场方向的应用,提高质粒的基因转移
在体外</em
DOI:
10.3791/3309-v
•
04:46 min
•
September 12, 2011
•
Mojca Pavlin
,
Saša Haberl
,
Matej Reberšek
,
Damijan Miklavčič
,
Maša Kandušer
1
Department of Fundamentals of Electrical Engineering, Mathematics and Physics
,
University of Ljubljana
,
2
Department of Biomedical Engineering
,
University of Ljubljana
Chapters
00:01
Title
00:04
Introduction
00:58
Cell Culture Preparation for the Experiment
01:42
Hardware Equipment
02:09
Gene Electrotransfer Protocol
03:31
Image Acquisition and Determination of Gene Electrotransfer Efficacy
Summary
Automatic Translation
English (Original)
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Automatic Translation
基因转染,电是提高约两次脉冲的应用过程中改变电场方向时,细胞活力不受影响。基因转染的增加是由主管DNA进入细胞的膜面积增加。
Tags
Electric Field
Gene Transfer
Plasmid Gene
In Vitro
Gene Electrotransfer
Cell Membrane
DNA Entry
Viral Vectors
Electric Pulse Protocols
Membrane Area
Gene Transfection
Green Fluorescent Protein
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