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GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

DOI:
10.3791/3314-v

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11:38 min

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October 24, 2011

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, ,
1DOE Plant Research Laboratory,Michigan State University, 2DOE Great Lakes Bioenergy Research Center,Michigan State University

Chapters

  • 00:05Title
  • 01:41Enzyme Production
  • 03:00Design of Experiment
  • 04:14Dispensing of Biomass and Enzymes
  • 07:03Determination of Glc and Xyl
  • 08:45Representative Results with GENPLAT
  • 10:27Conclusion

Summary

Automatic Translation

Automatic Translation

GENPLAT (GLBRC Enzyme Platform) is an automated platform for discovery and optimization of enzyme cocktails for biomass degradation. It can be adapted to multiple feedstocks and mixtures of enzymes containing multiple components.

Tags

GENPLATAutomated PlatformBiomass Enzyme DiscoveryCocktail OptimizationHigh Throughput PlatformLignocellulosic FeedstocksLiquid Transportation FuelsEnzymesSugars ReleasePretreatment/biomass CombinationsPichia PastorisTrichoderma ReeseiChromatographic PurificationSimplex Lattice Mixture ModelsRobotic Pipeting96-well FormatGlc And Xyl MeasurementEnzyme-linked Colorimetric AssaysOptimized Enzyme MixturesFeedstock And Pretreatment Combinations

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