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Patch-clamp Kapasitans Målinger og Ca 2 + Imaging ved Singel nerveterminaler i Retinal Slices
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
DOI:

09:16 min

January 19, 2012

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Chapters

  • 00:05Title
  • 01:37Preparation of Agar-embedded Retinal Slices
  • 04:21Electrophysiological Recordings and Ca2+ Imaging
  • 07:23Results: Identification and Ca2+ Imaging of Bipolar Cell Synaptic Terminals in Retinal Slices
  • 08:49Conclusion

Summary

Automatic Translation

Her beskriver vi en protokoll for utarbeidelse av agar-embedded retinal skiver som er egnet for elektrofysiologi og Ca2 + bildebehandling. Denne metoden gjør det mulig å studere bånd-type synapser i retinal microcircuits bruke direkte patch-clamp opptak av single presynaptiske nerveterminaler.

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