A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine,The University of Hong Kong, 2Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine,The University of Hong Kong, 3State Key Laboratory of Brain and Cognitive Sciences,The University of Hong Kong
Chapters
- 00:01Title
- 00:20Introduction
- 01:01Measure the INL thickness
- 08:20Quantify the RGC number
- 08:34Differentiate live and dead cells
- 12:56Measure a cell’s size
- 14:10Conclusion
Cette vidéo montre trois types d'analyses morphométriques de la rétine, qui comprennent la mesure de l'épaisseur de couche nucléaire interne, de quantifier le nombre de cellules ganglionnaires de la rétine (CGR) et de mesurer les tailles de CGR. La technique peut offrir une plate-forme simple mais scientifique pour analyses morphométriques.
Tags
Morphometric AnalysesRetinal SectionsRetinal DiseasesNeuronal CellsRetinal Ganglion Cell Layer (RGCL)N-methyl-D-aspartate (NMDA)-induced ExcitotoxicityRetinal Ischemia-reperfusion InjuryGlaucomaINL ThicknessInner Plexiform Layer (IPL) ThicknessCiticoline TreatmentKainic Acid-mediated Glutamate ExcitotoxicityRGC DensitySoma SizesElevated Intraocular PressureObjective MethodsEvaluating Retinal PathologiesTherapeutic StrategiesRetinal StructureMulti-layersDifferent Kinds Of NeuronsMorphometric ParametersCell NumberCell SizeThickness Of Different LayersCommercial Imaging SoftwarePrecise ValueAccurate CalculationChronic Glaucoma Model
