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Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
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Neuroscience
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JoVE Journal Neuroscience
Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

DOI:
10.3791/59694-v

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10:09 min

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June 07, 2019

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1Department of Anesthesiology,University of Virginia Health System, 2Department of Anesthesiology,Universita’ degli Studi di Padova

Chapters

  • 00:04Title
  • 01:27Post-fixation, Sequential Dehydration, and Staining of Brain Sections
  • 03:38Infiltration and Embedding
  • 05:01Flat Embedding
  • 06:09Capsule Embedding
  • 07:28Results: Electron Micrographs of Newborn Rat Brain Tissue
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

We describe procedures for processing newborn rat brain tissue to obtain high-resolution electron micrographs for morphometric analysis of synaptic vesicle distribution at nerve terminals. The micrographs obtained with these methods can also be used to study the morphology of a number of other cellular components and their dimensional structural relationships.

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Keywords: Newborn Rat BrainUltrastructural Morphometric AnalysisSynaptic Vesicle DistributionNerve TerminalsTransmission Electron MicroscopyOsmium TetroxidePhosphate BufferUranyl AcetateSynaptic FunctionSynaptic DevelopmentAnesthetics

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