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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Article DOI: 10.3791/3448-v • 10:59 min • February 27th, 2012
February 27th, 2012 •
1, 1, 1, 1
1Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée

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Summary
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We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.

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Quantitative Automated High-throughput Genome-wide RNAi Screens C. Elegans Gene Function Knockdown Bacteria DsRNA Libraries Functional Genomic Studies Laborious Methods Semi-automated Protocols Microscopic Imaging Image Analysis Robotic Handling COPAS Biosort MBioLIMS Data Management Sample Tracking Solid Medium Plates Host-pathogen Interactions Anti-fungal Innate Immunity
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