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Chapters
- 00:05Title
- 01:33Day 1, Preparation of 96-well NGM RNAi Plates, 96-DeppWell LB Plates, and RNAi Bacterial Clone Culturing
- 03:14Day 2, Seeding RNAi Bacterial Clones onto 96-well NGM RNAi Plates
- 04:46Days 3 and 4, RNAi Feeding of Worms and Infection of Worms with D. coniospora Spores
- 06:02Day 5, Observation and Storage of Plates Prior to Automated Quantitative Analysis
- 07:29Representative RNAi Results
- 10:27Conclusion
Vi beskriver en protokol med<em> C. elegans</em> Og RNAi fodring biblioteker, der tillader automatisk måling af flere parametre, såsom fluorescens, størrelse og opacitet af individuelle orme i en population. Vi giver et eksempel på et skærmbillede til at identificere gener involveret i anti-fungal medfødte immunitet<em> C. elegans</em>.
Tags
QuantitativeAutomatedHigh-throughputGenome-wideRNAi ScreensC. ElegansGene FunctionKnockdownBacteriaDsRNALibrariesFunctional Genomic StudiesLaborious MethodsSemi-automated ProtocolsMicroscopic ImagingImage AnalysisRobotic HandlingCOPAS BiosortMBioLIMSData ManagementSample TrackingSolid Medium PlatesHost-pathogen InteractionsAnti-fungal Innate Immunity
