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监测与固态纳米孔蛋白质吸附
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Monitoring Protein Adsorption with Solid-state Nanopores
DOI:

08:51 min

December 02, 2011

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Chapters

  • 00:05Title
  • 01:15Manufacturing Solid-state Nanopores in Silicon Nitride Membranes
  • 03:52Wetting of the Solid-state Nanopore
  • 06:18Monitoring Protein Adsorption
  • 07:12Typical Solid-state Nanopores
  • 08:15Conclusion

Summary

Automatic Translation

一个方法是使用固态纳米孔监测到无机表面蛋白质的非特异性吸附描述。该方法采用电阻脉冲的原则,允许实时和单分子水平探讨吸附。因为单一的蛋白质吸附过程是远离平衡,我们建议平行合成纳米孔阵列的就业,使表观一级反应速率常数蛋白质吸附定量测定以及和Langmuir吸附常数。

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