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Oprettelse Forbigående Cell membranporer Brug en Standard Inkjet printer
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Oprettelse Forbigående Cell membranporer Brug en Standard Inkjet printer

DOI:
10.3791/3681-v

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07:07 min

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March 16, 2012

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, , , ,
1Department of Bioengineering,Clemson University

Chapters

  • 00:05Title
  • 01:22Converting the HP DeskJet 500
  • 02:31Converting Stock HP Ink Cartridges (HP 26 Black Ink Cartridge)
  • 03:09Cleaning Ink Cartridge and Making Cell Suspension – “Bioink”
  • 03:59Bioprinting
  • 04:45Results: Representative Bioprinted Cell Images
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

En beskrivelse af de metoder, der anvendes til at konvertere en HP DeskJet 500 printer til en bioprinter. Printeren er i stand til at behandle levende celler, hvilket forårsager forbigående porer i membranen. Disse porer kan anvendes til at inkorporere små molekyler, herunder fluorescerende G-actin, i de udskrevne cellerne.

Tags

Transient Cell Membrane PoresInkjet PrinterBioprintingDirect Cell Application TherapiesBiosensor MicrofabricationThermal Inkjet PrintingGene TransfectionCell ViabilityFluorescently Labeled G-actin MonomersCell Plating MethodsManual MicroinjectionPore Size LimitationHP DeskJet 500 Printer

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