This content is Free Access.
Chapters
- 00:05Title
- 00:49Preparing the Cryopreservation Straws and Other Materials
- 01:42Mouse Sperm Cryopreservation
- 03:34Preparing for Sperm Recovery and IVF
- 04:33Mouse Sperm Recovery and IVF
- 06:05Cryopreservation and IVF Success
- 06:45Conclusion
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
Tags
Mouse Sperm CryopreservationI·Cryo KitGenetically Modified MiceTransgenesisKnockout TechnologiesCryopreservation Of EmbryosCryoprotective AgentRaffinoseSkim MilkArchiving And Distribution Of StrainsRecovery Of Valuable StrainsSperm MotilityProgressive MotilityInbred Mouse StrainsTwo-cell Stage Embryo DevelopmentIVFGM Mouse Lines
