/

/

在OVO在鸡胚视网膜电穿孔

JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

In Ovo Electroporation in Embryonic Chick Retina

Please note that all translations are automatically generated. Click here for the English version.

在OVO在鸡胚视网膜电穿孔

DOI:

•

06:15 min

•

February 05, 2012

•

Mohammed M. Islam, Sung Tae Doh, Li Cai²

¹Department of Pharmacology,University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, ²Department of Biomedical Engineering,Rutgers University

Chapters

00:05Title
00:56Egg Handling and Needle Preparation
03:20Injection and Electroporation

Summary

Automatic Translation

该视频的总体目标是显示如何执行有针对性的视网膜注射<em>在OVO</em> DNA / RNA的结构，在汉堡包和汉密尔顿22-23阶段，这是关于胚胎第4天（E4），成鸡胚胎视网膜电。这种技术是非常有用的，以研究基因表达，基因调控，并在发展中国家小鸡视网膜的形态学变化。

Tags

Ovo Electroporation Embryonic Chick Retina Retinal Development Gene Regulation Recombinant DNA/RNA Technology Reporter Gene Assay Gene Over-expression Gene Knock Down Targeted Retinal Injection Hamburger And Hamilton Stage 22-23 Embryonic Day 4 (E4)Green Fluorescent Protein (GFP)Subretinal Space Retinal Stem/progenitor Cells Late-born Neurons

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在OVO的HH阶段10个鸡胚Electroporations

整装在鸡的抗体染色方法

小鸡前OVO文化前OVO CAM含量：它是如何真正起作用

电子显微镜对胚胎的制备果蝇胚胎心管

原代培养及质粒电穿孔尔蒂小鼠器官。

利用鸡胚神经管破译基因定义的组的神经元轴突途径在OVO电穿孔

视频人类胚胎干细胞克隆生长的生物信息学分析

单细胞转染鸡胚

膜片钳和灌注技术研究离子通道的表达爪蟾卵母细胞

在体内电穿孔Morpholinos到成年斑马鱼视网膜

Read Article