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Lavmolekylært Protein berikelse på mesoporous silika Thin Films for biomarkører
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Chapters
- 00:05Title
- 01:31Chip Fabrication and Sample Pre-treatment
- 03:42Serum Fractionation
- 05:27MALDI-TOF Analysis of Peptides
- 06:40Data Analysis
- 08:36Results: iManipulation of MSP Thin Films for Selective Protein Enrichment
- 12:24Conclusion
Vi utviklet en teknologi basert på mesoporous silika tynn film for selektiv gjenvinning av lavmolekylære proteiner og peptider fra humant serum. De fysisk-kjemiske egenskapene til våre mesoporous chips ble finjustert for å gi betydelig kontroll i peptid berikelse og dermed profilere serum proteomet for diagnostiske formål.
Tags
Low Molecular Weight Protein EnrichmentMesoporous Silica Thin FilmsBiomarker DiscoveryCirculating BiomarkersEarly DiagnosisPrognosisTherapeutic EfficiencyLow Molecular Weight Proteome (LMWP)Mass Spectrometry (MS)Dynamic RangeProtein And Peptide ConcentrationsSample Pre-treatmentHighly Abundant ProteinsLow-abundance SpeciesProteomic-based ApproachesTwo-dimensional Polyacrylamide Gel-electrophoresis (2D-PAGE)Shotgun Proteomics MethodsClinical ApplicationsIsolation Of LMWP From BloodHigh Throughput ScreeningMulti-fractionation SystemMesoporous Silica Chips
