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Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
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Chapters
- 00:05Title
- 01:14Cloning of Recombinant Plasmids Encoding for aECM Proteins
- 03:59Bacterial Expression of aECM Proteins
- 05:35Lysis of Bacterial Cultures
- 07:04Purification of aECM Proteins Using Inverse Transition Cycling
- 08:42Results: Analysis of aECM Proteins
- 10:05Conclusion
Recombinant technologies have enabled material designers to create novel artificial proteins with customized functionalities for tissue engineering applications. For example, artificial extracellular matrix proteins can be designed to incorporate structural and biological domains derived from native ECMs. Here, we describe the construction and purification of aECM proteins containing elastin-like repeats.
