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JoVE Journal Bioengineering
Mikropipette Aspiration af substrat-vedhæftede celler til at estimere Cell Stivhed
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Mikropipette Aspiration af substrat-vedhæftede celler til at estimere Cell Stivhed

Article DOI: 10.3791/3886-v • 10:31 min • September 27th, 2012
September 27th, 2012 •
1, 1, 2, 1
1Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois, 2Institute for Medicine and Engineering, University of Pennsylvania

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Her beskriver vi en hurtig og enkel metode til at måle celle stivhed. Det generelle princip ved denne fremgangsmåde er at måle membran deformation som reaktion på veldefineret undertryk påføres gennem en mikropipette til celleoverfladen. Denne fremgangsmåde tilvejebringer et effektivt værktøj til at studere biomekaniske egenskaber af substrat-bundne celler.

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Bioengineering Biofysik Biomedical Engineering Medicine Cellular Biology Cell stivhed biomekanik microaspiration cellemembranen cytoskeleton
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