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基质贴壁细胞的微管吸吮的估计单元刚度
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
DOI:

10:31 min

September 27, 2012

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Chapters

  • 00:05Title
  • 01:45Preparation of Cells
  • 02:38Pulling Glass Micropipettes
  • 03:53Microaspiration and Image Acquisition
  • 07:17Analysis
  • 07:46Results: Factors That Control Membrane Deformability
  • 10:03Conclusion

Summary

Automatic Translation

在这里,我们描述了一个快速和简单的方法来测量细胞的刚度。这种方法的一般原则是响应于施加到细胞表面通过微量吸移管的良好定义的负压来测量膜变形。这种方法提供了一个功能强大的工具,研究基板贴壁细胞的生物力学特性。

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