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Bioluminescens Imaging af NADPH oxidase aktivitet i forskellige dyremodeller
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Chapters
- 00:05Title
- 02:09Animals Models: Intratracheal Administration of Zymosan
- 04:07Animal Models: Cecal Ligation and Puncture (CLP)
- 05:15Animal Models: Administration of Oral Carbon Tetrachloride
- 05:52Acquiring Bioluminescent Images
- 06:55Data Analysis using Region of Interest
- 07:45Detection of NADPH Oxidase Mediated ROS Production
- 09:57Conclusion
NADPH-oxidase er den vigtigste kilde af reaktive oxygenarter (ROS) i fagocytter. På grund af den flygtige karakter af ROS, er det vanskeligt at måle og overvåge ROS niveauer i levende dyr. Et minimalt invasiv metode til seriel kvantificering af ROS i levende mus er beskrevet.
Tags
Bioluminescence ImagingNADPH Oxidase ActivityAnimal ModelsAntibacterial DefenseAntifungal DefenseInflammatory ResponseReal-time KineticsROS GenerationResearch ToolHost DefenseInflammationInjuryChronic Granulomatous DiseaseCGDInherited DisorderNADPH Oxidase ComponentsGp91phox-deficient MiceP47phox-deficient MiceImpaired Host DefensePhagocytesIsoforms Of NADPH OxidaseQuantify ROS ProductionModels Of Inflammation And Injury
