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En Galvanotaxis Assay for Analyse af neurale Prækursormaterialer cellemigration Kinetics i en eksternt påført Direct Current Electric Field
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Neuroscience
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JoVE Journal Neuroscience
A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field

En Galvanotaxis Assay for Analyse af neurale Prækursormaterialer cellemigration Kinetics i en eksternt påført Direct Current Electric Field

DOI:
10.3791/4193-v

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11:00 min

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October 13, 2012

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, ,
1Institute for Biomaterials and Biomedical Engineering,University of Toronto, 2Lyndhurst Centre,Toronto Rehabilitation Institute, 3Department of Surgery,University of Toronto

Chapters

  • 00:05Title
  • 01:25Galvanotaxis Chamber Preparation
  • 05:30Live Cell Time-lapse Imaging
  • 08:55Kinematic and IHC Analysis of Differentiated and Undifferentiated NPCs
  • 10:35Conclusion

Summary

Automatic Translation

Automatic Translation

I denne protokol vi vise hvordan man opfører brugerdefinerede kamre, der tillader anvendelsen af ​​en jævnstrøm elektrisk felt for at aktivere time-lapse imaging af voksen hjerne afledt neural precursorcelletype translokation under galvanotaxis.

Tags

Galvanotaxis AssayNeural Precursor Cell MigrationExternally Applied Direct Current Electric FieldNeural Stem CellsNeuroregenerative StrategiesMobilization Of NPCsLesion SiteEndogenous PrecursorsPeriventricular RegionMechanisms Of NPC MigrationDirect Current Electric Fields (dcEFs)Galvanotaxis PhenomenonPhysiological Electric FieldsCell MigrationNormal DevelopmentWound Repair

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