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前全体体内培养，发展的果蝇大脑
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Neuroscience
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JoVE Journal Neuroscience
Ex vivo Culturing of Whole, Developing Drosophila Brains
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前全体体内培养，发展的果蝇大脑

DOI:
10.3791/4270-v

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08:39 min

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July 27, 2012

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, ,
1National Institute of Neurological Disorders and Stroke, 2National Human Genome Research Institute,National Institutes of Health, Bethesda, MD

Chapters

  • 00:05Title
  • 01:22Culturing Larval Brains
  • 02:23Culturing Pupal Brains
  • 03:30Fixing, Staining, and Mounting Larval and Pupal Brains
  • 04:38Representative Brain Culturing Results
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种方法，由哪一个可以模仿<em>在体内</em>发展<em>果蝇</em>在一个蘑菇体<em>体外</em>文化系统。

Tags

Ex Vivo CulturingDrosophila BrainsChronic Genetic ManipulationsCell BiologyDevelopmentCentral Brain StructuresPharmacological InterventionsLive ImagingSubcellular CompartmentAxon Initial Segment (AIS)Neuron-specific Cyclin-dependent KinaseCdk5Pharmacological InhibitorsActin OrganizationCell-surface Protein Fasciclin 2MutantsRemodelingConnectionsEmbryonic Mushroom Body (MB)MetamorphosisLarva To AdultOlfactory Learning And Memory

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