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<sub> 1</sub> F<sub> O</sub> ATPase Vehikelpræparationen og Teknik for Performing patchclamp Optagelser af Submitochondrial vesikelmembraner
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
DOI:

08:21 min

May 04, 2013

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Chapters

  • 00:05Title
  • 02:00Brain Mitochondrial Isolation
  • 04:04Submitochondrial Vesicles Isolation
  • 05:13Electrophysiological Recording
  • 06:35Results: Patch Clamp Recordings of Submitochondrial Vesicle Membranes
  • 07:46Conclusion

Summary

Automatic Translation

En fremgangsmåde til at isolere submitochondrial vesikler beriget i F1FO ATP synthase komplekser fra rottehjerne er beskrevet. Disse vesikler tillader undersøgelse af aktiviteten af ​​F1FO ATPase kompleks og dets modulation ved hjælp af teknikken af ​​patch clamp optagelse.

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