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<sub> 1</sub> F<sub> O</sub> ATPase Vehikelpræparationen og Teknik for Performing patchclamp Optagelser af Submitochondrial vesikelmembraner
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Chapters
- 00:05Title
- 02:00Brain Mitochondrial Isolation
- 04:04Submitochondrial Vesicles Isolation
- 05:13Electrophysiological Recording
- 06:35Results: Patch Clamp Recordings of Submitochondrial Vesicle Membranes
- 07:46Conclusion
En fremgangsmåde til at isolere submitochondrial vesikler beriget i F1FO ATP synthase komplekser fra rottehjerne er beskrevet. Disse vesikler tillader undersøgelse af aktiviteten af F1FO ATPase kompleks og dets modulation ved hjælp af teknikken af patch clamp optagelse.
Tags
F1FO ATPaseVesicle PreparationPatch Clamp RecordingsSubmitochondrial Vesicle MembranesMitochondriaATP ProductionOxidative PhosphorylationProgrammed Cell DeathF1FO-ATP SynthaseApoptosisNecrosisBCL-2 Family ProteinsMitochondrial Outer MembranePro-apoptotic FactorsCytosolNecrotic Cell DeathMatrix CalciumMitochondrial Permeability Transition Pore (mPTP)Inner Membrane PoreBcl-xL Protein
