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Treatment of Osteochondral Defects in the Rabbit’s Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots
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Treatment of Osteochondral Defects in the Rabbit’s Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Treatment of Osteochondral Defects in the Rabbit’s Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

DOI:
10.3791/4423-v

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11:22 min

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May 21, 2013

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, , , , , ,
1Department of Orthopaedic Sports Medicine,Klinikum rechts der Isar der Technischen Universität München, 2Department of Radiology,Klinikum rechts der Isar der Technischen Universität München, 3Institute of Experimental Oncology and Therapy Research,Klinikum rechts der Isar der Technischen Universität München, 4Department of Radiology,Uniklinik Köln

Chapters

  • 00:05Title
  • 01:36Preparation of a Donor Rabbit for the Isolation of Mesenchymal Stem Cells
  • 03:00Flushing of Rabbit MSC from Bones and Expansion
  • 06:09Preparation of Fibrin Clots in vitro
  • 07:57Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots
  • 09:32Results: Successful Isolation and Implantation of Allogeneic Mesenchymal Stem Cells into an Artificial Osteochondral Defect
  • 10:42Conclusion

Summary

Automatic Translation

Automatic Translation

An experimental technique for the treatment of osteochondral defects in the rabbit's knee joint is described. The implantation of allogeneic mesenchymal stem cells into osteochondral defects provides a promising development in the field of tissue engineering. The preparation of fibrin-cell-clots in vitro offers a standardized method for implantation.

Tags

Osteochondral DefectsRabbit’s Knee JointAllogeneic Mesenchymal Stem CellsFibrin ClotsArticular CartilageSubchondral BoneRestorationSurgical Treatment OptionsAutologous TransplantsAllogeneic TransplantsDonor Site Morbidity

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