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在文化序列富集的精原干细胞和祖细胞（SSCS）推导长期成年小鼠SSC线
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JoVE Journal Biology
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
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在文化序列富集的精原干细胞和祖细胞（SSCS）推导长期成年小鼠SSC线

DOI:
10.3791/50017-v

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12:26 min

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February 25, 2013

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,
1Department of Surgery,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 01:41Preparation of Feeder Cells
  • 03:54Dissociation of Mouse Testis Tissue to Obtain Donor Germ Cells
  • 06:17Plating Testicular Suspension and Colony Picking for First Passage
  • 09:00Subsequent Expansion and Passaging of SSCs by Trituration
  • 10:13Representative SSC Culturing Results
  • 11:51Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍一个简单的方法来获得和保持从成年小鼠精原干细胞和祖细胞株。该方法利用从体细胞室的成年小鼠睾丸的饲养层细胞。这种技术适用于常见的小鼠品系，包括转基因，基因敲除，基因敲除的小鼠。

Tags

Keywords: Spermatogonial Stem CellsSSCsAdult MouseIn Vitro CultureSerial EnrichmentProgenitor CellsTestisSelf-renewalDifferentiationNeonatalAdultSomatic CellsStem Cell NicheJK1 Feeder CellsPassaging

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