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生物质的使用限制金额高效的染色质免疫沉淀
JoVE Journal
Biology
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JoVE Journal
Biology
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Please note that all translations are automatically generated.
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生物质的使用限制金额高效的染色质免疫沉淀
DOI:
10.3791/50064-v
•
14:29 min
•
May 01, 2013
•
Dean Tantin
,
Warren P. Voth
,
Arvind Shakya
1
Department of Pathology
,
University of Utah School of Medicine
Chapters
00:05
Title
01:10
Preparation of Chromatin
03:58
Chromatin Immunoprecipitation (ChIP)
08:38
Analysis
12:04
Results: DNA Enrichment from Primary Lymphocytes using ChIP
13:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个可靠的方法,使用的主要的T细胞染色质免疫沉淀。该方法是建立在标准的方法,但使用一组特定的条件和试剂,提高效率有限的数量的细胞。重要的是,在数据分析阶段的详细描述。
Tags
Chromatin Immunoprecipitation
ChIP
DNA-protein Interactions
Transcription Factors
Histones
RNA Polymerase
DNA Repair
Quantitative Analysis
PCR
Standard Curves
Primer Design
Difficult-to-obtain Cell Types
Sensitivity
Reproducibility
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