Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
 

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

Article DOI: 10.3791/3257
November 12th, 2011

Chapters

Summary November 12th, 2011

Here we provide a protocol for culturing rat cortical neurons in the presence of a glial feeder layer. The cultured neurons establish polarity and create synapses, and can be separated from the glia for use in various applications, such as electrophysiology, calcium imaging, cell survival assays, immunocytochemistry, and RNA/DNA/protein isolation.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter