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Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
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Neuroscience
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JoVE Journal Neuroscience
Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

DOI:
10.3791/3257-v

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12:32 min

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November 12, 2011

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, ,
1Department of Pharmacology and Physiology,Drexel University College of Medicine

Chapters

  • 00:05Title
  • 01:18Glia Culture (2 Weeks Before Plating Neurons)
  • 05:17Secondary Glia Culture (48 Hours Before Plating Neurons)
  • 07:18Neuronal Dissection and Culture
  • 11:29Representative Results of Bilaminar Co-culture of Primary Rat Cortical Neurons
  • 12:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here we provide a protocol for culturing rat cortical neurons in the presence of a glial feeder layer. The cultured neurons establish polarity and create synapses, and can be separated from the glia for use in various applications, such as electrophysiology, calcium imaging, cell survival assays, immunocytochemistry, and RNA/DNA/protein isolation.

Tags

Bilaminar Co-cultureRat Cortical NeuronsGlial Feeder LayerExperimental NeedsGlass SubstratePlastic Growth SubstrateThermanoxAstroglia-enriched Secondary CultureNeuronal GrowthSurvivalDifferentiationTrophic FactorsBrain EnvironmentNeuronal-glial InteractionsGlia Contamination

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