Chemistry
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La producción de disulfuro estabilizados complejos transmembrana de péptidos de Estudios Estructurales
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Summary March 6th, 2013
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Estudios biofísicos y bioquímicos de las interacciones entre los dominios de la proteína de membrana integrados se enfrentan a muchos desafíos técnicos, la primera de las cuales es la de conseguir material de estudio adecuado. En este artículo se describe un protocolo para producir y purificar estabilizadas por puentes disulfuro complejos de péptidos transmembrana que son adecuados para el análisis estructural por la solución de resonancia magnética nuclear (RMN) y otras aplicaciones analíticas.
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