ना, कश्मीर और एच, कश्मीर ATPase में कटियन परिवहन मापने<em> Xenopus</em> परमाणु अवशोषण स्पेक्ट्रोफोटोमेट्री द्वारा oocytes: रेडियोआइसोटोप assays के लिए एक वैकल्पिक
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.