Medindo o transporte de catiões de Na, K e H, K-ATPase em<em> Xenopus</em> Oócitos por espectrofotometria de absorção atômica: uma alternativa para Ensaios de Radioisótopos
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.