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快速高通量直链淀粉测定冻干马铃薯块茎样品
 
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快速高通量直链淀粉测定冻干马铃薯块茎样品

Article DOI: 10.3791/50407
October 14th, 2013

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Summary October 14th, 2013

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Click here for the English version.

这个协议描述了通过一个依赖于碘和葡萄糖分子中淀粉的链之间的复合物的形成放比色法高。碘形成络合物与两个直链淀粉和支链淀粉中长链。加入碘的淀粉样品后,直链淀粉和支链淀粉的最大吸收出现在620和550nm处分别。直链淀粉/支链淀粉比例可以从620和550 nm处的吸光度值之比来估计并比较它们的标准曲线,其中特定的已知浓度作图的吸收值。这种高通量,廉价的方法可靠,重复性好,让土豆克隆的大量人口的评价。

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