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富亮氨酸的代谢标记重复激酶1和2与放射性磷酸盐

JoVE Journal
Biology

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JoVE Journal Biology

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

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富亮氨酸的代谢标记重复激酶1和2与放射性磷酸盐

9,897 Views

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11:31 min

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September 18, 2013

DOI:

10.3791/50523-v

DOI:

10.3791/50523-v

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11:31 min

September 18, 2013

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10 Views

Jean-Marc Taymans¹, Fangye Gao¹, Veerle Baekelandt¹

¹Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences,KU Leuven and Leuven Institute for Neuroscience and Disease (LIND)

Summary

富含亮氨酸的重复激酶1和2（LRRK1和LRRK2）是多结构域蛋白，其编码GTP酶都和激酶结构域和它们磷酸化的细胞中。在这里，我们提出了一个协议，以^32磷正磷酸盐标记LRRK1和LRRK2的细胞，从而提供衡量其整体的细胞磷酸化水平的一种手段。

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