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Journal
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Chemistry
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表面钝化单分子蛋白质研究
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
Surface Passivation for Single-molecule Protein Studies
Please note that all translations are automatically generated.
Click here for the English version.
表面钝化单分子蛋白质研究
DOI:
10.3791/50549-v
•
10:35 min
•
April 24, 2014
•
Stanley D. Chandradoss
,
Anna C. Haagsma
,
Young Kwang Lee
,
Jae-Ho Hwang
,
Jwa-Min Nam
,
Chirlmin Joo
1
Kavli Institute of NanoScience, Department of BioNanoScience
,
Delft University of Technology
,
2
Department of Chemistry
,
Seoul National University
Chapters
00:05
Title
01:35
Slide Preparation and Cleaning
04:08
Amino-silanization of Slides and Coverslips
05:24
Surface Passivation Using Polymer (The First Round)
07:30
Assembling a Microfluidic Chamber
08:52
Results: Quality Control
10:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了使用聚乙二醇(PEG)的钝化玻璃表面的方法。该协议涵盖了表面清洗,表面功能化和PEG涂层。我们引进用于治疗表面与PEG分子过两轮,比现有的方法而产生钝化优质的新战略。
Tags
Keywords: Single-molecule
Protein
Surface Passivation
Fluorescence Spectroscopy
PEG Coating
Surface Cleaning
Surface Functionalization
Piranha Etching
Surface Immobilization
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