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顺行运输Alphaherpes病毒传播的活细胞成像
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Immunology and Infection
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JoVE Journal Immunology and Infection
Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
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顺行运输Alphaherpes病毒传播的活细胞成像

DOI:
10.3791/50723-v

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15:31 min

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August 16, 2013

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, ,
1Department of Immunology and Infectious Diseases,Montana State University, 2Department of Molecular Biology,Princeton University

Chapters

  • 00:05Title
  • 01:27Environmental Control Conditions for Live-cell Fluorescent Microscopy
  • 03:44Real-time Imaging of Anterograde Virion Transport Events
  • 06:43Overnight Time-lapse Imaging of Anterograde Spread Events
  • 09:08Image Processing and Export
  • 10:48Results: Representative Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
  • 15:06Conclusion

Summary

Automatic Translation

Automatic Translation

活细胞成像的alphaherpes病毒感染使动态的定向运输和细胞间传播的事件分析。在这里，我们提出的方法，利用重组病毒株表达荧光融合蛋白，以方便在感染病毒组件可视化的初级神经元。

Tags

Anterograde TransportLive Cell ImagingFluorescent Fusion ProteinsViral Particle AssembliesCell-cell SpreadParticle Transport VelocitiesProtein LocalizationViral Genetic TechniquesAlphaherpes Virus

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