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Mikrofluidik-On-Chip-Capture-Cycloaddition reversibel Unbeweglichkeitseffekt kleine Moleküle oder Mehrkomponenten-Strukturen für Biosensor-Anwendungen
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
DOI:

14:43 min

September 23, 2013

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Chapters

  • 00:05Title
  • 01:48Preparation of GST and Nanoparticle Conjugates
  • 03:48Surface Preparation
  • 05:07Monitoring On-chip Capture-cycloaddition of Functionalized Molecules
  • 06:26Monitoring Immobilization Density and Determination of Cycloaddition Rates
  • 08:27Measuring the Binding of FKBP12 to Immobilized AP1497
  • 10:49Results: Real-time Monitoring of On-chip Small Molecule Immobilization by Surface Plasmon Resonance
  • 13:51Conclusion

Summary

Automatic Translation

Wir präsentieren eine Methode für die schnelle, reversible Immobilisierung von kleinen Molekülen und funktionalisierte Nanopartikel Baugruppen für die Oberflächenplasmonenresonanz (SPR)-Studien mit sequentiellen On-Chip-Cycloaddition bioorthogonale Chemie und Antikörper-Antigen-Capture.

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