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Mus embryoutvikling i en Serum-fri Whole Embryo Kultur System
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JoVE Journal Biology
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System

Mus embryoutvikling i en Serum-fri Whole Embryo Kultur System

DOI:
10.3791/50803-v

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08:55 min

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March 01, 2014

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,
1Neuroscience Division of the Biomedical & Health Sciences Institute,University of Georgia, 2Department of Cellular Biology,University of Georgia

Chapters

  • 00:05Title
  • 01:47Preparation
  • 02:41Collecting Embryos
  • 06:22Culturing Mouse Embryos
  • 07:37Cultured Mouse Embryos
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Serum benyttes i embryo kulturer inneholder ukjente komponenter som kan påvirke utfallet av eksperimenter, spesielt i studier som involverer signal interaksjoner. Her har vi benyttet en serum-fri oksygenrikt kultur-systemet og viser at midten av svangerskapet mus embryoer dyrket i 16-40 hr morfologiske utviklingen sammenlignes med embryoer utvikler seg i livmoren.

Tags

Keywords: Mouse Embryonic DevelopmentSerum-free CultureWhole Embryo CultureRolling Bottle CultureYolk SacOrganogenesisIn Vitro Development

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