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June 30, 2010
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Early embryonic lung organ culture is a very useful system to study epithelial-mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceeds under specific conditions that can be readily manipulated in this system.
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Mouse Embryonic Lung CultureMolecular MechanismsBranchingLung Primordial SpecificationBranching MorphogenesisPulmonary Cell LineagesLung EndodermSurrounding MesenchymeMesotheliumInductive SignalsEpithelial-mesenchymal-mesothelial InteractionsEmbryonic Lung Organ CultureEpithelial-mesenchymal InteractionsSerumless Culture MediaGain Of FunctionLoss Of FunctionExpressed ProteinsRecombinant Viral VectorsTransgenic Mouse StrainsAntisense RNARNA Interference Gene Knockdown
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Carraro, G., del Moral, P., Warburton, D. Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching. J. Vis. Exp. (40), e2035, doi:10.3791/2035 (2010).
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