Journal
/
Biology
/
Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

DOI:
10.3791/2035-v

•

07:32 min

•

June 30, 2010

•

, ,
1Saban Research Institute,Childrens Hospital Los Angeles

Chapters

  • 00:00Title
  • 00:39Introduction
  • 01:22Embryonic Lung Isolation
  • 05:22Embryonic Lung Culture
  • 06:21Representative Lung Cultures
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

Early embryonic lung organ culture is a very useful system to study epithelial-mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceeds under specific conditions that can be readily manipulated in this system.

Tags

Mouse Embryonic Lung CultureMolecular MechanismsBranchingLung Primordial SpecificationBranching MorphogenesisPulmonary Cell LineagesLung EndodermSurrounding MesenchymeMesotheliumInductive SignalsEpithelial-mesenchymal-mesothelial InteractionsEmbryonic Lung Organ CultureEpithelial-mesenchymal InteractionsSerumless Culture MediaGain Of FunctionLoss Of FunctionExpressed ProteinsRecombinant Viral VectorsTransgenic Mouse StrainsAntisense RNARNA Interference Gene Knockdown

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2035?article_type=v

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
check_url/2035?article_type=v

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
check_url/2035?article_type=v

Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI
check_url/2035?article_type=v

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
check_url/2035?article_type=v

A System for ex vivo Culturing of Embryonic Pancreas
check_url/2035?article_type=v

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
check_url/2035?article_type=v

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
check_url/2035?article_type=v

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
check_url/2035?article_type=v

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
check_url/2035?article_type=v

Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts

Read Article