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Mus embryonale udvikling i et serum-frit Hele Embryo Culture System
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JoVE Journal Biology
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System

Mus embryonale udvikling i et serum-frit Hele Embryo Culture System

DOI:
10.3791/50803-v

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08:55 min

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March 01, 2014

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,
1Neuroscience Division of the Biomedical & Health Sciences Institute,University of Georgia, 2Department of Cellular Biology,University of Georgia

Chapters

  • 00:05Title
  • 01:47Preparation
  • 02:41Collecting Embryos
  • 06:22Culturing Mouse Embryos
  • 07:37Cultured Mouse Embryos
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Serum i embryokulturer indeholder ukendte komponenter, der kan påvirke resultatet af eksperimenter især i undersøgelser med signalering interaktioner. Her udnyttede vi en serum-frit iltet kultur system, og vise, at midten af drægtigheden museembryoer dyrket for 16-40 hr udviser morfologiske udvikling sammenlignes med embryoner udvikler sig i livmoderen.

Tags

Mouse Embryonic DevelopmentSerum-free CultureWhole Embryo CultureRolling Bottle CultureYolk SacOrganogenesisIn Vitro Development

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