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In situ TEM of Biological Assemblies in Liquid
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Bioengineering
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JoVE Journal Bioengineering
In situ TEM of Biological Assemblies in Liquid

In situ TEM of Biological Assemblies in Liquid

DOI:
10.3791/50936-v

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08:28 min

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December 30, 2013

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, , , ,
1Applications Science,Protochips, Inc., 2Virginia Tech Carilion Research Institute

Chapters

  • 00:05Title
  • 01:49Preparation of Affinity Capture Devices
  • 03:25Capture Macromolecules
  • 04:40Assembly of the Microfluidic Chamber and Loading of the In situ Specimen Holder
  • 05:27Imaging in Liquid Using a Transmission Electron Microscope
  • 06:45TEM Accurately Displays Double-layered Particles at Near Atomic Resolution
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.

Tags

Keywords: In Situ TEMBiological AssembliesLiquid EnvironmentTransmission Electron MicroscopyMicrofluidic HolderSimian RotavirusDouble-layered ParticlesAffinity Biofilms3D Structure Determination

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