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Journal
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Immunology and Infection
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使用<em>大蜡螟</em>作为模式生物来研究<em>嗜肺军团菌</em>感染
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Use of
Galleria mellonella
as a Model Organism to Study
Legionella pneumophila
Infection
Please note that all translations are automatically generated.
Click here for the English version.
使用<em>大蜡螟</em>作为模式生物来研究<em>嗜肺军团菌</em>感染
DOI:
10.3791/50964-v
•
12:30 min
•
November 22, 2013
•
Clare R. Harding
,
Gunnar N. Schroeder
,
James W. Collins
,
Gad Frankel
1
Center for Molecular Bacteriology and Infection
,
Imperial College London
Chapters
00:05
Title
01:38
Preparation of
L. pneumophila
for Infection
03:04
Preparation and Infection of
G. mellonella
05:39
Analysis of Larval Mortality
06:17
Extraction and Processing of Hemolymph
08:57
Quantifying the Bacterial CFU
09:37
Virulence of WT and Dot/Icm-deficient
L. pneumophila
12:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
蜡蛾
蜡螟
的幼虫最近被确立为一种
体内
模型来研究
嗜肺军团菌
感染。在这里,我们展示了基本技术,
军团菌
的发病特点的幼虫,包括接种,细菌毒力和复制的测量和提取被感染的血细胞和分析。
Tags
Galleria Mellonella
Legionella Pneumophila
Infection Model
Hemocytes
Phagocytes
Dot/Icm Type IV Secretion System
Legionella Containing Vacuole
Bacterial Virulence
Competition Assays
Article
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