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Identifikation af protein-protein interaktion i<em> Drosophila</em> Voksen Heads af Tandem Affinitetsrensning (TAP)
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Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

Identifikation af protein-protein interaktion i<em> Drosophila</em> Voksen Heads af Tandem Affinitetsrensning (TAP)

DOI:
10.3791/50968-v

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10:36 min

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December 05, 2013

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, , ,
1Neuroscience Center of Excellence,Louisiana State University Health Sciences Center

Chapters

  • 00:05Title
  • 01:39Prepare Samples for Tandem Affinity Purification Procedure
  • 04:02Tandem Affinity Purification Procedure
  • 07:44Results: Identification of Interacting Proteins in the Drosophila Brain by Tandem Affinity Purification
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

Drosophila er berømt for sin kraftfulde genetisk manipulation, men ikke for sin egnethed dybdegående biokemisk analyse. Her præsenterer vi en TAP-baseret procedure til at identificere interagerende partnere i ethvert protein af interesse fra fluen hjernen. Denne procedure kan potentielt føre til nye muligheder for forskning.

Tags

Keywords: Protein-protein InteractionDrosophilaAdult HeadsTandem Affinity Purification (TAP)Mass SpectrometryGAL4/UAS SystemProtein ComplexGenetic ScreensBiochemical ScreensNeurobiology

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