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微创建立小鼠原位膀胱移植瘤的
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts
Please note that all translations are automatically generated.
Click here for the English version.
微创建立小鼠原位膀胱移植瘤的
DOI:
10.3791/51123-v
•
08:15 min
•
February 11, 2014
•
Wolfgang Jäger
,
Igor Moskalev
,
Claudia Janssen
,
Tetsutaro Hayashi
,
Killian M. Gust
,
Shannon Awrey
,
Peter C. Black
1
Department of Urologic Sciences
,
University of British Columbia
Chapters
00:05
Title
01:37
Preparation of Cell Lines
02:54
Preparation of Animals and Experimental Setup
04:14
Separation of Bladder Wall Layers and Intramural Inoculation of Bladder cancer Cells
05:54
Representative Bladder Cancer Injection Results
07:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
所建立的方法接种初级原位浸润性膀胱癌移植瘤需要剖腹探查和动员膀胱。此过程对其造成对小鼠显著发病率,在技术上是挑战性的和费时的。因此,我们开发了一种高精度,经皮穿刺的方法利用超声引导。
Tags
Orthotopic Bladder Cancer Xenografts
Minimally Invasive
Percutaneous Inoculation
Ultrasound Guidance
UM-UC1
UM-UC3
UM-UC13
Bioluminescence Imaging
Tumor Growth
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